Deveron Medical Practice in Banff has announced it is withdrawing from the GP contract with NHS Grampian.
There are currently more than six thousand patients registered locally.
The Aberdeenshire Health and Social Care Partnership has said that it is exploring alternative arrangements.
In the announcement of the withdrawal from the GP contract , the practice cited “ongoing difficulties” with recruiting doctors as the reason behind the move.
The situation has been repeated in other parts of the north-east – most recently in Torry and Rosemount in Aberdeen – and is down to a failure by the SNP government to address a shortage of family doctors.
Mr Duguid said: “There is no doubt where responsibly for this crisis in GP provision lies.
"This is a direct symptom of SNP mismanagement of the Scottish NHS.
"The Nationalists have been in charge of health for more than a decade, and are simply failing general practice. There have been warnings – most notably from the Royal College of GPs – of a looming crisis in general practice with shortages of new entrants to the profession and an ageing workforce. The Scottish Conservatives would ensure 11% of all NHS spending goes directly to general practice, making family doctors a priority and helping to boost working conditions, recruitment and retention. We also need more training places for medical students at our local universities. At the moment, aspiring doctors are being turned away because the SNP’s policy of free tuition has led to a cap on places for Scottish students”